hrp0084p3-828 | Fat | ESPE2015

Long-Term Effects of a Ketogenic vs a Hypocaloric Diet in Children and Adolescents with Obesity

Partsalaki Ioanna , Karvela Alexia , Spiliotis Bessie E

Background: The prevalence of childhood obesity is high worldwide and predisposes to adult obesity and metabolic disorders. Many dietary approaches have been proposed to reduce this prevalence, but no single diet has proven to be superior to others in terms of long-term weight loss maintenance in children.Objective and hypotheses: To evaluate and compare long-term body weight changes among obese children and adolescents who had lost at least 10% of their...

hrp0092p3-48 | Diabetes and Insulin | ESPE2019

Oral Glucose Tolerance Test (OGTT) as a Useful Tool for Early Diagnosis of Type 2 Diabetes Mellitus and Prediction of Metabolic Risks in Children and Adolescents

Kostopoulou Eirini , Tikka Maria , Rojas Gil Andrea Paola , Partsalaki Ioanna , Spiliotis Bessie

Objective: Type 2 Diabetes Mellitus(T2DM)and obesity represent two major health hazards in children and adolescents, with rising prevalence. Several markers have been developed in order to diagnose T2DM and detect potential metabolic abnormalities.The objective of the study was to examine glucose tolerance in Greek children and adolescents and the differences in the glucose, insulin and c-peptide response curves between male and female children and adolescents...

hrp0092p3-68 | Diabetes and Insulin | ESPE2019

Seasonal Variation and Epidemiological Parameters in Children from Western Greece with Type 1 Diabetes Mellitus (T1DM)

Kostopoulou Eirini , Papachatzi Eleni , Skiadopoulos Spyros , Rojas-Gil Andrea-Paola , Spiliotis Bessie

Introduction: A positive correlation between the onset of T1DM and winter has been suggested by studies conducted in different countries.Objective: To investigate the seasonal variation of T1DM diagnosis and additional epidemiological parameters in children from Western Greece diagnosed with T1DM.Methods: 105 patients, 44 males and 61 females, aged 1 to 16 years (mean: 8.2±4 y...

hrp0089fc12.2 | Diabetes and Insulin 2 | ESPE2018

Apoptosis and Oxidative Stress Markers During the Oral Glucose Tolerance Test (OGTT)

Gil Andrea Paola Rojas , Katsa Maria Efthymia , Tzortzis Nomikos , Ioannidis Anastasios , Kostopoulou Eirini , Spiliotis Bessie E

Background: In adults, insulin resistance is associated with vascular damage and progressive loss of the endothelial protective functions. Additionally, it further complicates the micro- and macro- vascular environment through increasing oxidative stress and inflammation.Aim: The purpose of this study was to evaluate how apoptosis and antioxidant markers are correlated with biochemical markers in children, during the glucose tolerance te...

hrp0082p1-d2-114 | Fat Metabolism & Obesity (1) | ESPE2014

Pro-Inflammatory (M1) and Anti-Inflammatory (M2) Profiles in Adipose Tissue of Lean and Obese Children and Adolescents

Karvela Alexia , Avgeri Aikaterini , Vlotinou Eleni D , Georgiou George , Papachristou Dionysios J , Spiliotis Bessie E

Background: Childhood obesity predisposes to metabolic disorders. Low grade inflammation of adipose tissue (AT) associated with macrophage infiltration may lead to metabolic complications. Two macrophage activation states M1 (pro-inflammatory) and M2 (anti-inflammatory) exist in AT with surface markers CD40 (M1), CD206 (M2) and CD163 (M2). M1 polarization correlates with metabolic complications.Objective and hypotheses: To study the expression of CD40, C...

hrp0082p2-d1-363 | Fat Metabolism & Obesity | ESPE2014

Novel Uncoupling Protein 1 Expression in White Adipocytes of Subcutaneous Abdominal Adipose Tissue in Children and Adolescents: A Protective Mechanism for Metabolic Equilibrium?

Karvela Alexia , Avgeri Aikaterini , Vlotinou Eleni D , Georgiou George , Papachristou Dionysios J , Spiliotis Bessie E

Background: Morbid childhood obesity predisposes to metabolic disorders such as diabetes type 2. In mice, heat-producing ‘brown-like’ (beige) adipocytes can suppress weight gain and metabolic disease through the action of uncoupling protein 1 (UCP1) localized in the mitochondria.Objective and hypotheses: To study the expression of UCP1 in the adipose tissue of lean&obese children and adolescents.Method: Paraffin embed...

hrp0084fc8.2 | Obesity - Basic | ESPE2015

Apoptosis Inhibitor of Macrophages: an Anti-Inflammatory Adipocyte Factor in Mild Adolescent Obesity?

Karvela Alexia , Avgeri Aikaterini , Vlotinou Eleni D , Georgiou George , Papaxristou Dionysios , Miyazaki Toru , Spiliotis Bessie E

Background: Adipose tissue (AT) in obesity is characterized by low grade inflammation. The apoptosis inhibitor of macrophages (AIM; also called CD5L) is incorporated into adipocytes leading to increased lipolysis. Excess AIM-dependent lipolysis induces adipose tissue macrophage recruitment. M1 (proinflammatory) macrophage infiltration, with surface marker CD40, correlates with metabolic complications.Objective and hypotheses: To study serum levels and AT...

hrp0084p2-402 | GH & IGF | ESPE2015

The Involvement of the Epidermal Growth Factor Receptor in the Successful GH Signalling and the Role of p21 in the Negative Regulation of the GH/GHR and EGF/EGFR Pathways, in GH Transduction Defect

Kostopoulou Eirini , Gil Andrea Paola Rojas , Karvela Alexia , Spiliotis Bessie Eugenia

Background: GH transduction defect (GHTD) is characterised by severe short stature and impaired STAT3 phosphorylation, which is overcome by simultaneous induction of GHTD fibroblasts with 200 ng/ml GH and short interference mRNA CIS (GH200/siRNA) or with 1 000 ng/ml GH (GH1000) and is clinically expressed with ‘catch-up’ growth after rhGH treatment.Objective and hypotheses: The involvement of epidermal growth factor receptor (EGFR) in the succe...

hrp0084p2-404 | GH & IGF | ESPE2015

The Role of β-TrCP, an E3 Ubiquitin Ligase, in the Signalling of the GH and Epidermal Growth Factor Pathways in Growth Hormone Transduction Defect

Kostopoulou Eirini , Gil Andrea Paola Rojas , Karvela Alexia , Spiliotis Bessie Eugenia

Background: epidermal growth factor (EGF) stimulates cell growth and differentiation through its receptor EGFR. Cross-talking between the GH and EGF signaling pathways is important for normal cellular development. GH transduction defect (GHTD), a clinical disorder characterized by impaired STAT3 phosphorylation due to excessive GHR degradation, is caused by over-expression of the E3 ubiquitin ligase, CIS.Induction of GHTD fibroblasts with 200 ng/ml hGH (GH200) and silencing mR...

hrp0094p1-69 | Diabetes B | ESPE2021

Oxidized lipid-associated protein damage in children and adolescents with type 1 diabetes mellitus: new diagnostic/prognostic clinical markers.

Kostopoulou Eirini , Kalaitzopoulou Electra , Papadea Polyxeni , Skipitari Marianna , Gil Andrea Paola Rojas , Spiliotis Bessie , Georgiou Christos ,

Background: Type 1 diabetes mellitus (DM1), a chronic metabolic disorder of autoimmune origin, has been associated with oxidative stress (OS), which plays a central role in the onset, progression and long-term complications of DM1. The markers of OS lipid peroxidation products, lipid hydroperoxides (LOOH), and also malondialdehyde (MDA) and thiobarbituric reactive substances (TBARS) that oxidatively modify proteins (Pr) (i.e., PrMDA and PrTBARS, respectively),...